A study conducted in Serrana, a small town in São Paulo state used as a model for COVID-19 vaccination in Brazil, shows that mass vaccination reduced the severe case and death rates even while the gamma and delta variants were circulating. Gamma and delta were considered alarming because they spread so much faster than previous variants.

Based on an analysis of the virus’s evolutionary history (phylogeny), the researchers showed that the dynamics of SARS-CoV-2 substitution in the town was similar to the pattern seen in the rest of Brazil. The ancestral strains (B.1.1.28 and B.1.1.33) were replaced by gamma, delta (first detected in India in 2020 and originally labeled B.1.617.2) and more recently omicron. In Serrana, however, the study showed that most cases caused by all three variants (88.9%, 98.1% and 99.1% respectively) were mild, thanks to immunization with CoronaVac (Sinovac Biotech-Butantan Institute). Coverage had then reached 80% of the target population.

The phylogenetic analysis was applied to 4,375 genomes obtained between June 2020 and April 2022, the period between the introduction of SARS-CoV-2 and completion of the double-dose vaccination process.

According to the authors, their genomic surveillance exercise not only monitored the spread of the key variants in the town but also helped identify some rare variants of interest, such as C.37, which circulated in the Andean countries but was under-represented in Brazil, and alpha, which was detected in Serrana but did not spread elsewhere. All told, the scientists detected 52 sublineages of SARS-CoV-2 in the town.

An article on the study is published in the journal Viruses. Codenamed Project S, the study was conducted by Butantan Institute with FAPESP’s support. The authors are researchers affiliated with Butantan Institute, the Ribeirão Preto Blood Center and the Center for Cell-Based Therapy (CTC), a Research, Innovation and Dissemination Center (RIDC) funded by FAPESP and hosted by the University of São Paulo’s Ribeirão Preto Blood Center and Medical School (FMRP-USP).

Serrana was a model for a clinical study of the first mass vaccination of a town’s entire adult population (over 18) using CoronaVac before the Ministry of Health began official vaccine rollout. A large-scale sequencing program was developed to analyze all SARS-CoV-2 positive samples obtained in the town in real time (read more at: revistapesquisa.fapesp.br/en/a-town-turned-laboratory/).

“Next-generation sequencing is increasingly affordable and widely used for genetic monitoring of infectious diseases and the viral variants involved. The technologies now available are applicable even in relatively remote areas with scant resources. This type of surveillance helps us prevent outbreaks, understand how novel viral genotypes spread and identify emerging viruses. Detection of novel agents and viral variants provides key input for public health decisions to avoid future pandemics or epidemics,” Svetoslav Nanev Slavov, first author of the article and a researcher at Butantan Institute, told Agência FAPESP.

Results

The 4,375 genomes analyzed in the study broke down as follows by variant: 1,653 delta (37.8%), 1,053 gamma (24.1%), 1,513 omicron (34.6%), 75 zeta (1.7%), and 81 other (1.9%). Most subjects were in the 21-50 age group, although there were participants of all ages.

According to the authors, based on the clinical scores of the subjects who tested positive, vaccination reduced morbidity and mortality in Serrana, especially during the gamma and delta waves. They compared the results with those of São José do Rio Preto, a medium-sized city about 200 km away, where mortality was higher during the gamma wave, especially among unvaccinated young people.

The beneficial effects of vaccination have been observed in other studies, showing that fully vaccinated people are less likely to contract symptomatic or asymptomatic infections. The conclusion is that vaccination reduces infection rates, numbers of severe cases and death rates due to SARS-CoV-2.

“In addition to genomic monitoring of patients who tested positive for SARS-CoV-2 in Serrana, the study demonstrated the benefits of early mass vaccination in significantly reducing morbidity and mortality due to this viral agent,” said Simone Kashima, last author of the article and a researcher at the Ribeirão Preto Blood Center.

The study serves as a basis for future research on the genetic monitoring of viral diseases and measures to combat them, she added.

The study was also supported by FAPESP via seven other projects (21/11944-6; 20/10127-1; 20/06441-2; 17/26950-6; 19/22155-2; 18/15826-5 and 14/50947-7).

About São Paulo Research Foundation (FAPESP)

The São Paulo Research Foundation (FAPESP) is a public institution with the mission of supporting scientific research in all fields of knowledge by awarding scholarships, fellowships and grants to investigators linked with higher education and research institutions in the State of São Paulo, Brazil. FAPESP is aware that the very best research can only be done by working with the best researchers internationally. Therefore, it has established partnerships with funding agencies, higher education, private companies, and research organizations in other countries known for the quality of their research and has been encouraging scientists funded by its grants to further develop their international collaboration. You can learn more about FAPESP at www.fapesp.br/en and visit FAPESP news agency at www.agencia.fapesp.br/en to keep updated with the latest scientific breakthroughs FAPESP helps achieve through its many programs, awards and research centers. You may also subscribe to FAPESP news agency at https://agencia.fapesp.br/subscribe